Control elements of the P2 promoter of the Antennapedia gene.
نویسندگان
چکیده
Antennapedia (Antp), a homeotic gene of Drosophila required for proper differentiation of the thorax of the fly, is expressed in complex spatial patterns during development. The gene is greater than 100 kb long and has two independently regulated promoters. To characterize cis-acting regulatory elements responsible for the expression pattern, fusions of the Antp promoter 2 cap site and upstream sequences to an Adh-lacZ gene were introduced into flies. A 10-kb sequence directs beta-galactosidase production in a pattern that closely resembles the endogenous P2 pattern. Transcription from the 10-kb fusions is regulated by three genes that regulate Antp transcription. Control elements, including a target of action of homeo-domain-containing proteins, were mapped by deleting parts of the 10-kb sequence.
منابع مشابه
Positive and negative control of the Antennapedia promoter P2.
To understand the nature of the regulatory signals impinging on the second promoter of the Antennapedia gene (Antp P2), analysis of its expression in mutants and in inhibitory drug injected embryos has been carried out. The maternally-active gene osk is identified as one of two general repressors of P2 which prevent Antp transcription until division cycle 14. Products of the zygotically-active ...
متن کاملTransvection and silencing of the Scr homeotic gene of Drosophila melanogaster.
The Sex combs reduced (Scr) gene specifies the identities of the labial and first thoracic segments in Drosophila melanogaster. In imaginal cells, some Scr mutations allow cis-regulatory elements on one chromosome to stimulate expression of the promoter on the homolog, a phenomenon that was named transvection by Ed Lewis in 1954. Transvection at the Scr gene is blocked by rearrangements that di...
متن کاملبررسی ارتباط بین ژنهای القایی عامل کروم سنسینگ وابسته به پروموتور و مقاومت به متی سیلین در سویههای استافیلوکوک اورئوس بالینی
Background and Objective: Quorum sensing (QS) phenomenon, promoters involved in this pathway and the effect of these promoters on the expression of some structural genes, such as agrA and PII, could reveal the proper activity and gene expression of antibiotic resistance in S. aureus. The aim of this study was defining the relationship between promoter-dependent Quorum Sensing induced genes and ...
متن کاملComparison of Promoter Sequences of Flowering Control Genes, FT1 and Three Versions of VIN3, in Susceptible and Resistant Sugar Beet Genotypes to Bolting
Autumn sowing of sugar beet is a suitable way in sustainable agriculture. Bolting is an undesirable phenomenon which reduces sugar beet yield and it is the most important limiting factor in autumn sowing of sugar beet. Identification and comparison of the sequence of flowering genes in various genotypes can help to understand the molecular mechanisms controlling bolting. In the previous studies...
متن کاملGenetic polymorphisms in the promoter region of catalase gene, creates new potential PAX-6 and STAT4 response elements
Catalase (CAT, OMIM: 115500) is an endogenous antioxidant enzyme and genetic variations in the regulatory regions of the CAT gene may alter the CAT enzyme activity and subsequently may alter the risk of oxidative stress related disease. In this study, potential influence(s) of the A-21T (rs7943316) and C-262T (rs1001179) genetic polymorphisms in the CAT promoter region, using the ALGGEN-PROMO.v...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Genes & development
دوره 2 12A شماره
صفحات -
تاریخ انتشار 1988